The lipids of whole blood. 1. Lipid biosynthesis in human blood in vitro

Human whole-blood granulocyte aggregation in vitro.

1. Aggregation assays are a commonly used technique for the study of granulocyte activation. These studies are usually performed using a pure cell suspension in buffer. This necessitates a separation procedure which is time-consuming and may modify the function of the cells. Interaction between different cell types is precluded. 2. To avoid these disadvantages a method was developed which quant...

the effect of e-64 on the developmental competence of sheep cocs during in vitro maturation

in the present study, the effect of e-64 at different concentrations (0.5, 1, 5 and 10 µm) added to (1) the ivm medium on oocyte nuclear maturation and developmental competence of ovine oocytes, and (2) to the ivc medium on embryonic development of ovine embryos were investigated.

The lipids of whole blood. 2. The exchange of lipids between the cellular constituents and the lipoproteins of human blood.

In Part 1 (James, Lovelock & Webb, 1959) and in the earlier work of Altman, Watman & Solomon (1952), it was reported that the cellular constituents of the blood are able to incorporate activity from sodium [Me-14C]acetate into their lipid components. It was also shown that the radioactive lipids synthesized by the cells are transferred during the course of incubation in vitro to the plasma lipo...

In vitro activity of actinospectacin in human whole blood.

A method for testing antibacterial substances in whole blood is described. The test agent for the method was actinospectacin which reportedly has good in vivo activity, approximately in the range with chloramphenicol, but relatively poor in vitro activity in the common media. In human whole blood, however, the in vitro activity compares favorably with chloramphenicol thus indicating that whole ...

The Human Whole Blood/il-1 in Vitro Pyrogen Test (wb/il-1)